MLT 2402: Microbiology I: Describe the colony and microscopic morphologies of various unknown microorganisms

Unknown Labs

Session Objectives:

By the end of this laboratory session, the student shall be able to:

  • Describe the colony and microscopic morphologies of various unknown microorganisms
  • Perform available biochemical tests on various unknown microorganisms
  • Determine the identity of an unknown microorganism using appropriate algorithms presented in class
  • Perform and/or assure that all appropriate QC was conducted on the used biochemistry kits and recorded in the QC workbook.
  • Follow all safety precautions that should be observed when working with the chemical and biological substances used in this laboratory experience

Virtual Lab Instructions

  1. Explain the principle behind catalase and coagulase test?
  • You are provided with a mixed culture of organism. Provide a flow chart to show different procedure involved in the identification process.
  • Explain a rapid biochemical test for the identification of Staphylococcus aureus?
  • Explain the principle of nitrate reduction test?
  • While performing the gram stain technique, you noticed that the culture contains gram negative rods and gram negative cocci. Explain in detail about your further proceedings of the experiment?
  • Explain the following:
  1. Alpha hemolysis
    1. Beta hemolysis
    1. Gamma hemolysis
  • A bacterium grown in the starch agar plate is suspected to have the enzyme amylase. How will you confirm the presence of amylase in the medium?
  • Explain the different biochemical tests of bacteria that rely on the fermentation of carbohydrates?
  • Escherichia coli has the ability to grow on the EMB agar plate, but Staphylococcus aureus cannot. Explain the reason?
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