• This practical report based on the results obtained from the “Whole genome sequencing for

• This practical report based on the results obtained from the “Whole genome sequencing for
identification and gene function prediction of bacterial genomes” pracs.
• Total marks available are 25
• You can submit a single Word Document/PDF file.
• The report should contains around 1500 words (excluding references) and contains the
following sections:

o Introduction
Include the following: 1) Application of whole genome sequencing in epidemiology
studies 2) What is spotty liver disease (SLD) in chickens and what bacteria is
responsible for the cause of SLD? 3) The aim of the practicals.
Material and Methods
Briefly describe the material and methods used in the pracs (for making libraries and
Bioinformatics), appropriately cite the sources of the methods
Results presentations includes 1) name of species; 2) ANI value; 3) Number of coding
sequences and RNAs of your isolate ; 4) virulence genes ( you need to write from the
data and research out about some virulence for example: motility, invasion, ,) 5)
antibiotic resistance genes 6) plasmids ; 7) Subsystem Feature Counts (SEED Viewer)
Interpretation of your findings, compare them with the literature: Is it the same species as
the bacteria known to cause SLD in chickens? What are the genomics and proteomics
features of the isolate and compare them to those of other common Campylobacter
species. The risk of antibiotic resistant plasmids present in bacteria isolated from poultry
sources. Here you need to write mor to show your understanding.
Write a short paragraph on the overall findings
All references mentioned in the text are correctly cited with consistent reference style for
every reference

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