Lab Topic 11: Forensic Application of Molecular Genetics

4. In Figure 11.1 in the lab manual; the Sample 2 individual (with regards to one STR locus) has 12 repetitions on one chromosome and 27 repetitions on the other chromosome (as shown by the two different bands at those locations in the gel). This makes them heterozygous at that location, so this could be written as (12,27). In the same Figure 11.1, why does the Sample 1 individual only have one band at the location in the gel representing 6 repetitions? 


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5.  In Table 11.1 in the lab manual, which of the missing persons (#1 or #2) can be eliminated as not being the body that was found, and why? (please read the paragraph before the table for important background information pertaining to the table)


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6. In Figure 11.3 in the lab manual; based upon your analysis of the gel results in that figure, which of the two potential fathers (#1 or #2) cannot be the father of that child, and why? 

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8. Why is it important to use this many locations, and not just consider a match at one loci to be sufficient to prove guilt in a criminal case?

9. Table 11.4 in the lab manual, shows the allele repetition frequency of several STR loci in certain species of cats. Based upon your analysis of the numbers in that table, what percentage of Persian cats would be expected to be (3,23) at STR Locus 3?


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10. Table 11.5 in the lab manual, shows the allele repetition frequency of several STR loci in human caucasians. Based upon your analysis of the numbers in that table, what percentage of those individuals would be expected to be (6,6) at STR Locus 2?


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