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Western Kentucky University Melting point determination and Thin Layer Chromatography Lab

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Table of physical constants (5 points): (tabulate name, structure, molar mass, density (liquids only), melting point, boiling point, and safety hazards for all chemicals mentioned in the procedure) 

Observations: (Include pictures of TLC plates)

Tabulated data (10 points): (construct a table including name of compound or over-the-counter drug, distance spot traveled, distance solvent traveled, calculated Rf values for each spot visualized, and color change in iodine chamber) 

Answer the questions below (5 points each):

  • Do your TLC results match the expected contents for each over-the-counter drug? Explain how your tabulated TLC data provides evidence to support each of your claims.
  • Explain any anomalies that you observed in your TLC plates (e.g., streaking or large spots).
  • How does the melting point of the mixture compare with the melting points of the pure compounds? Are these differences expected? Explain your answer.
  • Why is it important that spots are completely dry before developing the TLC plate? What might happen if you developed your TLC with wet spots? 
  • How could you use a mixed melting point to identify an unknown compound among several candidate molecules?

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