I’m working on a biochemistry multi-part question and need an explanation and answer to help me learn.
The first peptide is IEGKADST. Please draw the structure (pH=7) and indicate the
different possible y and b daughter ions that may result from running tandem MS-MS on
the structure. What is the sequence of the y3 and b6 ions?
b. The second peptide MSYGRLISKAETLARAGW is cleaved by the reaction with trypsin.
Then the products of the digestion are subjected to ESI-TOF MS in positive ion mode with
the peptides in a pH 2 solution. What peptide fragments will result and what are their m/z?
c. For the products of the digestion in question 2b, which product will reach the detector
first? Explain why? The peptide KTDLRFQSSAVMALQEACEAY is digested with thermolysin. Then the
products of the digestion are subjected to ESI-TOF MS in positive ion mode with the
peptides in a pH 2 solution. What peptide fragments will result and what are their m/z?
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