TU Enzyme Kinetics Effect of Substrate on Enzyme & Carbohydrates Lab Report

Enzyme Kinetics Lab Instruction and Answer Sheet

Answer the following questions about the results of this lab activity. Record your answers in the boxes with the questions. It is important to discuss how changes in absorptance over time explain the activity of an enzyme.

Send your completed lab report to your instructor.Don’t forget to save your lab report to your computer!

The relative rate of enzyme activity can be calculated with the following formula:

Spectrophotometer absorptance1 – Spectrophotometer absorptance2 = Relative rate of activity

Send your completed lab report to your instructor.Don’t forget to save your lab report to your computer!

Activity 1 – Effect of Substrate on Enzyme Activity

Answer the following questions about the results of this lab activity. Record your answers in the boxes with the questions.

1. Explain how differences in the relative rate of activity can be used to determine the specificity of an enzyme for a substrate.

1. Explain which carbohydrate is most specific for alpha-galactosidase based on your data.

1. Hypothesize why two or more molecules can be used as a substrate for one enzyme.

Activity 2 – Distinguishing cell types and Activity

Answer the following questions about the results of this lab activity. Record your answers in the boxes with the questions.

1. Explain which pH is optimal for alpha-galactosidase activity with a starch substrate.

1. Draw a graph of your data below using the Chart feature in the Insert tab of Word. The graph should have relative activity on the Y-axis and pH on the X-axis.

1. Hypothesize how a particular pH may cause an enzyme to best less effective at carrying out chemical reactions.

Activity 3 – Effect of Temperature on Enzyme Activity

Answer the following questions about the results of this lab activity. Record your answers in the boxes with the questions.

1. Explain which temperature is optimal for alpha-galactosidase activity with a starch substrate.

1. Draw a graph of your data below using the Chart feature in the Insert tab of Word. The graph should have relative activity on the Y-axis and temperature on the X-axis.

1. Hypothesize how a particular temperature may cause an enzyme to best less effective at carrying out chemical reactions. Hypothesize what the graph would show if more data points were taken for temperature at the optimum range.