Test Microbiology

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just two Lectures 12,13

study guide

Clone vs strain.

Phenotypic Identification Methods, what they are and what they tell you about the organism

Serology: Know how it is used in identifying bacteria cells and antigen antibody interaction

What the ELISA test is and what it is used for

Know what PCR is used for (not how it works, just its use)

Know that every species of bacteria can be identified by its nucleotide sequence within the

hypervariable region of 16 rRNA.

Rickettsia (slide 3, 4, 5 bolded info)

Bordetella pertussis: how it affects lungs to cause the disease (slide 7)

Gonorrhea: definition (slide 11)

Understand that asymptomatic gonorrhea allows the carrier to become a reservoir that allows spread and increased complications

LPS triggers an intense inflammatory response which is responsible for the cell and tissue

damage and discharge (that’s it, nothing more detailed than that)

It is growing resistant to most known antibiotics and potentially becoming untreatable

Nongonococcal Urethritis

C. trachomatis causes the most frequently reported STI in the United States, Chlamydia Burkholderia:

Adapted to live in a wide variety of environments

Causes nosocomial infections

It is responsible for outbreaks of infection due to contamination of disinfectants and medical solutions

Pseudomonas aeruginosa: slide 31, 32 bolded,

It is associated with biofilm formation in the lungs of Cystic Fibrosis patients.

Understand why it is so difficult to control its presence in hospitals and why it is difficult to treat-Slide 35

Enterics: The mechanism by which diarrhea/dysentery is caused by Shigella and Cholera

Know how stomach acid affects the ability of organisms to cause gastrointestinal disease

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