The goal of this project is to get the oxygen dependence of the triplet formation (Intersystem Crossing) within a dye by using lifetimes. You will be using the Rhodamine 6G that is in the door of the 4 °C Fridge in sample prep. Utilize the small cuvettes and dilute the sample to an appropriate concentration, I will let you decide that.
You will need to figure out a way to vary the concentration of oxygen in the solution, with some form of control. I would recommend using a nitrogen tank and a needle to flow N2 into the cuvette with different times and then capping it as quickly as you can. Another way is to this in the glove box, but that would require more time and add difficulty. You could also vary the amount of oxygen scavenging that you add to the sample to form basically a titration curve of it. So you have a few options to work with there.
I would also like you to a little lit review, basically find some articles and send me a quick summary of info you can find on the topic. Just to give you and idea and a starting point I linked the Wikipedia below, it does an okay job and shows a good Morse potential of what is happening here. The libre texts also has a good review intro on the luminescence.
0 comments