DNA Sequencing is a technique used to identify the genetic sequences of DNA and RNA. Protein sequencing can also be performed using mass spectrometry or a protein sequenator.
This assignment is designed to introduce you to the BLAST: Basic Local Alignment Search Tool, which is a powerful tool for molecular biologists and biochemists. Running BLAST searches allows you to input a genetic or protein sequence and determine what gene or protein it is… For microbes, you can also use it to determine what organism the sequence is coming from. For a tutorial on how to perform this assignment (provided by Johns Hopkins), please watch this video (8 min long). It’s from 2010, but the search parameters are basically the same…
identify the results of several genes that your friend Inigo Montoya submitted for sequencing yesterday. Several of the genes are from humans, but some others are from microbes (either bacteria or virus).
Here are the sequences:
Nucleotide sequences (use nucleotide blast)
Sequence A (from human)
Sequence B (from human)
Sequence C (from human)
Sequence D (from microbe)
Sequence E (from microbe)
Protein sequences (use protein blast)
Sequence F (from human)
Sequence G (from human)
Sequence H (from human)
Sequence I (from microbe)
Sequence J (from microbe)
You need to submit an assignment report containing the following information:
- From humans (A-C, F-H), the correct identification of the gene or protein that the sequence came from and what disease this gene or protein is associated with.
- From microbes (D,E,I,J), the correct identification of the organism the sequence came from.
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