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Princeton University Growth and Reproduction in Prokaryote Questions

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I’m working on a genetics discussion question and need an explanation to help me learn.

Growth and Reproduction in Prokaryote

Questions

  • Calculate the growth rate and generation time of a culture that increases in the exponential phase from 5 x 102 to 1 x 108 cells in 12 hours.
  • If the generation time is 90 min and the initial population contains 103 cells, how many bacteria will there be after 8 hours of exponential growth?

Essay response:

3. In terms of counting cells, what does a plating method accomplish that an electronic cell counting method does not?

4. Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?

5. Some hyperthermophiles can survive autoclaving temperatures. Are they a concern in health care?

6. People who use proton pumps inhibitors or antacids are more prone to infections of the gastrointestinal tract. Can you explain the observation considering what you have learned?

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