here is the grading rubric out of 100 points.
1. find an article describing a use of the polymerase chain reaction (10 points)
2. summerize the article in a 1-page (maximum) typed report, including: (40 points)
– who did the PCR? I.e medical group, research group, etc
describe what was the overall purpose of the PCR. for example:
were they trying to find out if a certain DNA existed in a sample?
were they trying to quantify the amount of DNA in one sample to another?
were they trying to mpdify / mutate a known DNA sequence?
– Think about the application and suggest what would be some of their bigest concerns in setting up and performing the PCR. for example:
did they know the sequence of the DNA they wanted to amplify?
would there be a problem with competing sequences? (was the target DNA pure and isolated or in a mixture of other DNAs?
Would there be a large concern of contaminating one sample withe another? that is, are they trying to determine presence/absence of a sequence in multiple samples or trying to quantify the amount of DNA present in one sample vs another?
– summerize their results. what did they find out or accomplish with the PCR tha was described in the article?
3. prepare a short power point presentation of the above (3-5 minutes) (40 points)
4. provide the link of the article you used (10 points)
time needed is at 11:00 AM PST. i have no extension for this. please commit before agreeing. graduates or anyone in the field of biochem or biotechnology is preferred
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