I have three HW and each one is very short. The med micro is about 10 questions. And the unknown is like another 10.
1. Using the given link to an article and using other online sources, explain what Guillain-Barre syndrome (GBS) is and what the symptoms are. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC28159…
2. Using the same article from #1, What are lipooligosaccharides (LOS) and how does LOS cause Guillain-Barre syndrome or GBS?
3. Based on the tabulated information above, enter the five-digit codes for the three organisms:
E. coli ________________ E. aerogenes __________________ K. pneumoniae _______________
4. The Path-CHEK system that we use to detect coliforms has a sensitivity of 13 colony forming units per centimeter squared. What does sensitivity mean? What does having a sensitivity of 13 CFU/cm2 mean in relation to interpreting a negative result?
5. Using online sources, list other detection systems that are being used to monitor coliforms in the environment such as water reservoirsMedical Microbiology Homework
Name: ____________________________________________
1a. Based on the results you recorded in Table 2 and compared to the data in Table 1, what Lancefield group is S. pyogenes in? What are the major features of this organism that places it in this group?
1b. What major diseases are caused by this organism? Does it occur as normal flora, and if so, where in the body does it reside?
2a. Based on the results you recorded in Table 2 and compared to the data in Table 1, what Lancefield group is S. agalactiae in? What are the major features of this organism that places it in this group?
2b. What major diseases are caused by this organism? Does it occur as normal flora, and if so, where in the body does it reside?
3a. Based on the results you recorded in Table 2 and compared to the data in Table 1, what Lancefield group is S. pneumoniae in? What are the major features of this organism that places it in this group?
3b. What major diseases are caused by this organism? Does it occur as normal flora, and if so, where in the body does it reside?
4a. Based on the results you recorded in Table 2 and compared to the data in Table 1, what Lancefield group is S. mitis in? What are the major features of this organism that places it in this group?
4b. What major diseases are caused by this organism? Does it occur as normal flora, and if so, where in the body does it reside?
5a. Based on the results you recorded in Table 2 and compared to the data in Table 1, what Lancefield group is E. faecalis in? What are the major features of this organism that places it in this group?
5b. What major diseases are caused by this organism? Does it occur as normal flora, and if so, where in the body does it reside?
Unknown 1
OBSERVATIONS:
How long did growth take to appear?
Is the growth typical of bacteria (smooth and paste-like) or mold (hairy and filamentous), or do you see both? if you grew mold, try to ignore it since it is not from your body!
Focusing on the bacterial colonies only, based on differences you see in relation to colony size, color, shape, surface attributes (like smooth, shiny, wrinkles, for example), determine the number of different species you have and record here.
Do you have good isolation of the different species in your sample (colonies of the different types that are not touching)? You may have some species that are well-isolated while other are not, please note any differences you see.
Using the lab manual to identify the correct terminology for colony description, describe one well-isolated colony (or, if you didn’t get good isolation then note the color and surface features only). Write NA if you did not get isolated colonies.
IF you didn’t get growth on some or all of your plates, explain what you think are the possible reasons why you didn’t get growth.
Tell us about your overall experience—
Were you successful? (how do you know?)
Were there any things you would do differently?
Did you make mistakes?
Did your agar crack?
Did you make improvements after the first plate you inoculated–what adjustments did you make?
Did you follow the steps in the right order, or did you forget anything?
Please share any final thoughts about this experience of streak plating.
You must include, along with this completed document, pictures of your labeled plates (your labels must be clear and visible in the pictures) BEFORE incubation (growing them).
You must include pictures of those same plates (your labels must be clear and visible in the pictures) AFTER incubation (growing them). Make sure that the growth is visible in the pictures if growth occurs. Include pictures of ALL plates, even the ones that did not grow any microbes.
PROPER PLATE DISPOSAL—once you make your observations and take your photographs, wrap up your plates so they cannot be opened, put in your ziplock bag and place in another bag and tape that bag shut. Place it in a secure outdoor trash bin so that no person or animal can get into them!
Figures from https://greenbioresearch.com/culture-bacteria-usin…
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