If we you have two proteins in a mixture, one with a pI of 4.5, one with a pI of 9.5. In order to prevent these molecules from interacting would you use high salt or low salt buffer and why? and what if you want to encourage interactions?
If we you have two proteins in a mixture, one with a pI of 4.5, one with a pI of 9.5. In order to prevent these molecules from interacting would you use high salt or low salt buffer and why? and what if you want to encourage interactions?
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