Drug dose and lc50

2. (Drug dose-response)
You are given a dataset for cell numbers from each of three hematopoietic stem
cell and progenitor subpopulations in response to different concentrations of a
putative actomyosin force inhibiting drug. Given that IC50 (lethal concentration) for this drug is
8uM:

i)
Plot absolute cell number (linear scale) vs drug dose (log scale) and determine
an appropriate curve fit for each of the three subpopulations – provide an
equation with numbers.

ii)
Determine IC50 for each curve. Is IC50 for each curve consistent with the known
IC50 for this drug?

iii)
Determine Hill’s co-efficient for each curve. What does this co-efficient tell
us about the drug’s mechanism of action?

ii)
Is this a ‘good’ drug to modulate any of the three subpopulations? Why or why
not? Explain in terms of IC50 and possible off-target effects.

HSC, MPP, and CPP are types of cells and so these need plots and lc50 relative to drug dose.