The lab notebook report for mode #2 analysis of your unknown insert should include: the mode of analysis (PCR/digestion analysis) you chose to evaluate the vector/insert, a detailed procedure of how all data that would have been obtained (demo data will be used), how the demo data is interpreted, and a final conclusion as to how your analysis of the demo data allowed you to confirm the insert assigned. Any graphs, tables, and photos must be included in your typed report and should be labeled so that they can be referenced in the conclusion of the report. Note that you do not need to include the data from analysis #1 in this report only discuss your finding in relation to your demo data from analysis #2. Remember you need to include all of your demo data in your lab report. Your report must contain the following data: Gel Photo from restriction analysis or PCR analysis of vector/insert, an overview of the procedure used to analyze your clone and a discussion of what you were testing for that would allow you to confirm the identity of you insert and rule out the possibility that it could have been the other insert that was provided.
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